October 20, 2023/Cancer/Research

The Role of Immune Escape in Post-Transplant Leukemia Relapse

Immunological pressure driving genetic mutations

23-CNR-4044609-CQD-Hero-650×450 AML study

A new study offers potential insights into the genetic underpinnings of post-transplant leukemic relapse in a large cohort of patients. The findings, published in Nature Communications, are the latest from the lab of Cleveland Clinic hematologist and lead author Jaroslaw Maciejewski, MD, PhD, whose groundbreaking research efforts over the past two decades continue to decode the complex molecular and genetic mechanisms of myelodysplastic syndromes (MDS) and myeloid neoplasia (MN).


Cleveland Clinic is a non-profit academic medical center. Advertising on our site helps support our mission. We do not endorse non-Cleveland Clinic products or services. Policy

Allogeneic bone marrow transplant (BMT) is the only curative option for patients with high-risk acute myeloid leukemia (AML) and MDS. This treatment exerts a graft-versus-leukemia (GvL) effect, in which the donor-derived immune response may both eliminate residual leukemia cells and decrease the likelihood of post-transplant relapse. However, some patients relapse anyway.

“In some cases, we know that recurrence is due to disease with high molecular risk features, in which we see the exact recapitulation of the initial myeloid drivers,” says Carmelo Gurnari, MD, hematologist and physician scientist at Cleveland Clinic Cancer Center and Tor Vergata University of Rome, and author of the study. “This is not true for every case, and we wanted to better understand why this transplant failure might be happening for some patients.”

Targeting human leukocyte antigen (HLA)

Earlier work from Dr. Maciejewski’s lab has demonstrated that genomic aberrations in the human leukocyte antigen (HLA) region may represent one of the possible forms of clonal hematopoiesis in aplastic anemia, whereby these alterations rescue the hematopoietic stem cell from the autoimmune attack. In the context of AML and MDS, the idea is that the same patterns of mutations could enable leukemic escape from immune surveillance. Dr. Gurnari, in conjunction with colleague and study co-first author Simona Pagliuca, MD, PhD, decided to investigate the role of HLA heterogeneity in disease recurrence after BMT.


“We undertook this broad immunogenetic study in hopes that we could find targets for future translation to the clinical setting,” says Dr. Pagliuca, who was a research fellow at Cleveland Clinic Lerner Institute during the study period and is now a hematologist and physician-scientist at Nancy University Hospital in France.

Researchers examined a cohort of 494 patients with AML, MDS and myeloproliferative neoplasms (MPN) who had received BMT at a median age of 59 years, 29 percent of whom experienced disease recurrence.

“We developed a new bioinformatic pipeline that is able to detect somatic mutations and losses in HLA class I and II,” says Dr. Gurnari. “Indeed, we found that the majority of mutations we saw were in post-transplant samples, and not in samples of patients who had undergone chemotherapy. Furthermore, this was happening at a later time point after transplant, indicating that immunological pressure was driving the mutations and the relapse.”


Researchers also calculated HLA evolutionary divergence, a new immunological measure that captures the level of antigen-immune system interaction in order to estimate its impact in this setting. They performed longitudinal immunogenomic and transcriptomic analysis in a subgroup of patients (N = 55) to broadly characterize potential features of immune dysfunction.

“Given their impact on response to post-transplant immunomodulation, these genomic aberrations can potentially help us to make decisions about post-relapse treatments,” says Dr. Gurnari, who is now studying how the same HLA aberrations may impact leukemic progression in MDS, thanks to an EvansMDS Young Investigator Award. Dr. Pagliuca has received multiple grants from the AA MDS International Foundation and Association pour la Recherche sur le Cancer for her investigations on HLA. The pair has worked together in Dr. Maciejewski’s lab and earlier this year published with him in Leukemia on the molecular landscape of immune pressure and escape in aplastic anemia.

Related Articles

Doctors working on MGUS screening study
March 18, 2024/Cancer/Research
Pilot Study Aims for Early Identification of Multiple Myeloma Precursor Among Black Patients

First-of-its-kind research investigates the viability of standard screening to reduce the burden of late-stage cancer diagnoses

Physician with patient
March 6, 2024/Cancer/Research
Targeting Uncontrolled Erythrocytosis in Polycythemia Vera with Rusfertide

Study demonstrates ability to reduce patients’ reliance on phlebotomies to stabilize hematocrit levels

Doctor measuring patient's waist size
February 26, 2024/Cancer/Research
Impact of Obesity on GVHD & Transplant Outcomes in Hematologic Malignancies

Findings highlight an association between obesity and an increased incidence of moderate-severe disease

Physician with patient
February 21, 2024/Cancer/Research
Strategies for Improving Clinical Trial Equity

Cleveland Clinic Cancer Institute takes multi-faceted approach to increasing clinical trial access

How antibody drug conjugates work
February 13, 2024/Cancer/Research
Real-World Use of Trastuzumab Deruxtecan

Key learnings from DESTINY trials

February 1, 2024/Cancer/Research
Possibilities of CRISPR Technology (Podcast)

Gene editing technology offers promise for treating multiple myeloma and other hematologic malignancies, as well as solid tumors

Disparities in multiple myeloma
January 25, 2024/Cancer/Research
Major Study Identifies Global Disparities in Drug Toxicity for Multiple Myeloma Treatment

Study of 401,576 patients reveals differences in cancer burdens as well as overall survival

Dr. Shilpa Gupta
December 27, 2023/Cancer/Research
A New Standard Emerges in Advanced Urothelial Carcinoma After Decades of First-Line Chemotherapy

Enfortumab plus pembrolizumab reduced risk of death by 53% compared with platinum-based chemotherapy